Sequencher

Sequencher is used for viewing, editing and assembling chromatograms.

Please read the following before downloading!

In order to run Sequencher properly, both the KeyAccess software and the Sequencher software must be downloaded and installed. KeyAccess must be installed first and configured before installing the Sequencher software.

There is a two hour time limit per user for Sequencher. If a few more hours are needed, check to see if another “key” is available by relaunching the Sequencher software. If no key is available, you will be put in a waiting queue for the next available key.

For large jobs that may require up to 24 hours use, please send an email to dna-seq@illinois.edu with your request and also the IP address of the computer that will be running Sequencher. An IP address is required for access to the 24 hour queue.

KeyAccess Installation for Windows

Choose the KeyAccess file for your operating system: K2Client32bit.exe K2Client-x64.exe

• Double-click on the downloaded KeyAccess file (K2Client.exe) to begin the installation process. “Run” the file.
• Under “Host DNS Name or IP Address,” type keyserver.biotech.illinois.edu, Click “Next.”

• Keep default “Basic client”; choose “Next.”
• Choose “Install,” then “Finish.”
• You will be asked to restart your computer.

Sequencher 5 Installation for Windows

Download Sequencher 5.4 for Windows here: Sequencher_5.4.exe

• Double-click on the downloaded file. “Run” the file.
• Follow the prompts, clicking “Next,” “I accept the terms,” “Next,” “Install,” “Finish.”

The Sequencher software will be found in Start>All Programs>Gene Codes>Sequencher 5.4.

When you launch the software, a dialog box will come up that gives you License Options. Choose “I have a license file,” “Next.”

Download the license key file here: Browse to the place where you saved the license key file. Click “Next.”

Click “Finish” and restart your computer.

KeyAccess Installation for Mac

• Launch the K2client file and follow the prompts: "Continue," "Install."
• When asked for the KeyServer Address, type keyserver.biotech.illinois.edu.

Keyserver Address

• You will then have to restart your computer.

Sequencher 5 Installation for Mac

• Download Sequencher 5.4 for Mac here: Sequencher for Mac

• Follow the prompts, "Continue," "Continue," "Agree," "Select the disk," "Continue," "Install."

• You may get a dialog box asking you to continue to install a Sentinel System Driver. "X" out of this dialog box, as it is not necessary to install it.

Sentinel System Driver

In Applications, in the Sequencher 5.4 folder, launch Sequencher.

You will get a dialog box with License Options.

License Options

Choose "I have a license file," "Continue."

Download this license file: Licensekey.lic

Browse to the locationn where you saved the file.

License file location

You will be informed that the license file has been installed, and to restart your computer.